Assessment expression of Serum Amyloid A and LncRNAAF085935 in patients with Hepatocellular Carcinoma, Hepatitis B and Hepatitis C Virus

Document Type : Review Articles

Authors

1 General Manager of School of Medicine BUC,Lecturer of Hospital Administration, MMA

2 Medical Biochemistry and Molecular Biology department, Faculty of medicine, Cairo University.

3 Endemic medicine and Hepatogastroenterology, Faculty of medicine, Cairo University

4 Medical microbiology and Immunology department, Faculty of Medicine for Girl, AL- Azhar University

5 Clinical and Chemical Pathology Department, Consultant Clinical Pathology, Cairo University Hospitals, Cairo, Egypt

Abstract

Long non coding RNAs (Lnc RNAs) and serum Amyloid A (SAA) have been suggested to have a role in hepatocarcinogenesis. In this work, to investigated the role of lnc RNA AF085935 and SAA as diagnostic biomarkers in sera of HBV, HCV, HCC patients. Subjects and Methods: HBV genotyping was assessed by. Lnc RNAAF085935 quantitative gene expression in whole blood by real time PCR and the SAA expression was measured using ELISA. 149 adult Egyptian subjects were divided into 35 HCC patients on top of HCV and 24 HCC patients on top of HBV, 35patients with HBV, 35 patients with HCV and 20 controls. Results: HBV genotype D was found in all of the samples. In HCC patients, HCV patients, and HBV patients; the levels of LncRNA-AF085935 and SAA were significantly upregulated than in healthy controls. (p value = <0.001). In addition, higher expressions of both LncRNA-AF085935 and SAA (p value = <0.001) were observed in HCC/HBV and HCC/HCV patients than in HCV or HBV patients. Conclusion: Lnc RNA-AF085935 and SAA were observed with an aberrant level in sera of HCC, HCV and HBV patients. Lnc RNA-AF085935 and/or SAA can be considered to be used as potential biomarkers in HCC.

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